Simultaneous recording of filtration and respiration in marine organisms in response to short‐term environmental variability

Climate change imposes unusual long-term trends in environmental conditions, plus some tremendous shifts short-term variability, exerting additional stress on marine ecosystems. This paper describes an empirical method that aims to improve our understanding of the performance benthic filter feeders experiencing changes such as temperature, time scales minutes hours, especially during daily cycles or extreme events heatwaves hypoxic upwelling. We describe Fluorometer and Oximeter equipped Flow-through Setup (FOFS), experimental design, methodological protocols evaluate flood data, enabling researchers monitor important energy budget traits, including filtration respiration filter-feeders response fine-tuned variability. FOFS allows online recording deviations chlorophyll dissolved oxygen concentrations induced by study organism. Transparent data processing through Python scripts provides possibility adjust procedures needs when working different contexts (e.g., temperature vs. pH, salinity, oxygen, biological cues) with filter-feeding species. successfully demonstrate functionality responses Baltic Sea blue mussels (Mytilus) one-day thermal cycles. practically a tool help exposing organisms variability for weeks months, relate observed responses, explain their findings potential generalize patterns. The method, therefore, more detailed description stress-response relationships detection species' tolerance limits.